Background: |
proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. |
Applications: |
WB, IHC, IF |
Name of antibody: |
SRC (Phospho-Tyr529) |
Immunogen: |
Synthetic peptide of human SRC (Phospho-Tyr529) |
Full name: |
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (Phospho-Tyr529) |
Synonyms: |
ASV; SRC1; c-SRC; p60-Src |
SwissProt: |
P12931 |
IHC positive control: |
Human breast carcinoma |
IHC Recommend dilution: |
50-100 |
WB Predicted band size: |
60 kDa |
WB Positive control: |
HT29 cells untreated or treated with PMA |
WB Recommended dilution: |
500-1000 |
IF Positive control: |
Hela cells |
IF Recommended dilution |
100-200 |