中文名稱: 兔抗RELA (Phospho-Ser276) 多克隆抗體
英文名稱: Anti-RELA (Phospho-Ser276) rabbit polyclonal antibody
別 名: p65; NFKB3
相關類別: 一抗
儲 存: 冷凍(-20℃) 避光
宿 主: Rabbit
抗 原: RELA (Phospho-Ser276)
反應種屬: Human, Mouse, Rat
標 記 物: Unconjugate
克隆類型: rabbit polyclonal
技術規格
Background: |
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. |
Applications: |
WB, IHC, IF |
Name of antibody: |
RELA (Phospho-Ser276) |
Immunogen: |
Synthetic peptide of human RELA (Phospho-Ser276) |
Full name: |
v-rel reticuloendotheliosis viral oncogene homolog A (avian) (Phospho-Ser276) |
Synonyms: |
p65; NFKB3 |
SwissProt: |
Q04206 |
IHC positive control: |
Human breast carcinoma |
IHC Recommend dilution: |
50-100 |
WB Predicted band size: |
65 kDa |
WB Positive control: |
Hela cells |
WB Recommended dilution: |
500-1000 |
IF Positive control: |
Hela cells |
IF Recommended dilution |
100-200 |