中文名稱: NIH/3T3細胞裂解液
英文名稱: NIH/3T3 Cell Lysate
別 名: NIH/3T3細胞裂解液
相關類別: 抗原
儲 存: 冷凍(-20℃) 避光
概述
NIH/3T3 cell lysates from Mouse fibroblasts cells were cultured in the media containing 10% FBS.
技術規(guī)格
技術規(guī)格
Background |
Cells were lysed in lysis buffer (50mM Tris(PH7.4), 150mM NaCl, 1% NP-40, 1% SDS). 100 μg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer. |