中文名稱: 231細(xì)胞裂解液
英文名稱: 231 Cell Lysate
別 名: 231細(xì)胞裂解液
相關(guān)類別: 抗原
儲 存: 冷凍(-20℃) 避光
概述
231 cell lysates from human breast cancer cells were cultured in the media containing 10% FBS.
Background
Cells were lysed in lysis buffer (50mM Tris(PH7.4), 150mM NaCl, 1% NP-40, 1% SDS). 100 μg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer.
技術(shù)規(guī)格